PHOTOELECTRIC STUDY OF SOME FACTORS RELATED TO BLOOD CLOTTING

Photoelectric Study of Some Factors Related to Blood Clotting.

The determination of the thrombic or prothrombic activity of blood or blood plasma preparations is beset with many difficulties. The use of a variety of methods and means of expressions of thrombin units has served to complicate rather than clarify the basic aspects of this problem. The application of existing assay methods to new prothrombin-containing systems likewise presents new problems. T...

Photoelectric determination of plasma clotting times.

A photoelectric clot timer is described. The apparatus allows a large analytic capacity, with good precision and correlation to manual methods, and can be used with capillary blood. Prothrombin analysis according to the method of Quick and Owren, the Thrombotest method, and determinations of partial thromboplastin time are applicable to the apparatus. The semiautomated procedure is extremely si...

Nomenclature for blood clotting factors.

THE FLOCCULATION MAXIMUM (pH) OF FIBRINOGEN AND SOME OTHER BLOOD-CLOTTING REAGENTS. (RELATIVE TURBIDIMETRY WITH THE EVELYN PHOTOELECTRIC COLORIMETER)

By means of a novel adaptation of the Evelyn photoelectric colorimeter to the measurement of relative turbidities, the question of the flocculation maximum (F.M.) in acetate buffer solutions of varying pH and salt content has been studied on (a) an exceptionally stable prothrombin-free fibrinogen and its solutions after incipient thermal denaturation and incomplete tryptic proteolysis, (b) plas...

Blood clotting factors in cerebrospinal fluid.

The activity of blood clotting factors has been investigated in cerebrospinal fluid. No cephalin-like activity was found in cerebrospinal fluid but prothrombin activity averaged about 0.5% of normal plasma activity. The activity of factor VII was negligible in almost all cases. The activity of antihaemophilic factors showed great variation but in the majority of cases it did not exceed 1% of no...